We will examine the methods that increase success in in vitro fertilization (IVF) under several headings:
With this method that increases success in IVF, after DNA amplification, structural and numerical abnormalities on chromosomes can be detected using a computer system by comparing it with a second DNA known to be normal. The sequences of the DNA fragments that undergo various processes are individually read according to each embryo and evaluated with a special algorithm.
The advantages of NGS over a-CGH method are as follows:
It is a technique that works with higher sensitivity due to its direct analysis method.
While the rate of detecting mosaic embryos with a-CGH is 5%, with NGS, this rate is between 15-30% (even 12% mosaicism can be observed in embryos that reach the blastocyst stage).
In ‘normal’ embryos identified by NGS, the abortion rate is lower compared to other PGS methods.
Higher implantation (clinical pregnancy) rates are achieved compared to the a-CGH method.
PGS is mostly applied in cases of:
Repeated IVF attempts (> 3)
Advanced maternal age (> 37 years)
Severe male factor
Patients with repeated pregnancy losses (> 3)
In conclusion, by using the mentioned genetic screening methods, selective embryo transfer is achieved, resulting in significantly increased pregnancy rates.